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pBJ1-human b2m

Plasmid

#12099

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

human beta-2-microglobulin (B2M Human)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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EC67904

Plasmid

#209470

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70189

Plasmid

#209450

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70197

Plasmid

#209458

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP beta (LAP)

Plasmid

#12557

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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EC67908

Plasmid

#211795

Purpose

Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

HptII, Cas12a, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mTagBFP2

Plasmid

#96935

Purpose

Retroviral plasmid containing mTagBFP2 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Tag Blue Fluorescent Protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP mCherry

Plasmid

#45235

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, mCherry

Tags

full length CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-LacZshRNA

Plasmid

#73977

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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EC67894

Plasmid

#209505

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA2 TKIT

Plasmid

#169442

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMGA

Plasmid

#139931

Purpose

M. magneticum/E. coli shuttle vector (pMGT and p15A ori)

Depositor

Christopher Voigt

Article

Schuerle et al ACS Synth Biol. 2020 Feb 21;9(2):392-401

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23119

Available Since

March 3, 2024

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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543-p3E-2A-mcherrypA

Plasmid

#26031

Depositor

Nathan Lawson

Article

Villefranc et al Development. 2013 Apr;140(7):1497-506. d

Insert

mCherry

Use

Gateway Cloning

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDB070.iodo.5

Plasmid

#99397

Purpose

Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)

Expression

Bacterial

Promoter

PN25, Ptet, and PproK

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Actin-OptoNB

Plasmid

#159595

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

ActinNB with AsLOV2 at loop 5 with 62-666

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pYMA1_SidK_mCH

Plasmid

#184906

Purpose

Legionella pneumophila SidK (1-278) - mCherry

Depositor

John Rubinstein

Article

Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

Insert

SidK (1-278) (sidK Legionella pneumophila)

Tags

mCherry

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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