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pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

Plasmid

#192822

Purpose

Golden Gate compatible plasmid with mCherry and Neo

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

mCherry and Neo

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

Plasmid

#223770

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Mutation

R108E/R125E

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Human, Synthetic)

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2557-2853)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Foxo1

Plasmid

#118588

Purpose

Expresses Foxo1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Foxo1 (Foxo1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Plasmid

#217511

Purpose

KD Recombinase dependent conditional smGdP-10XHA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Tags

smGdP-10XHA

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT L576P

Plasmid

#118848

Purpose

Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT L576P (KIT Human, Zebrafish)

Use

Zebrafish expression, tol4

Mutation

KIT L576P

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-1.7kbfabp6

Plasmid

#159087

Purpose

p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Insert

1.7kb fabp6 promoter (fabp6 Zebrafish)

Use

Promoter fragment 5' entry vector for tol2 c…

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_AroLITE

Plasmid

#215640

Purpose

For overexpression of Gal4-DBD FOXA3 AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 AroLITE (FOXA3 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_AroPERFECT

Plasmid

#215641

Purpose

For overexpression of Gal4-DBD FOXA3 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 AroPERFECT (FOXA3 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

Plasmid

#134611

Purpose

Mammalian expression of mouse KIF5A 378-1027

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5A (Kif5a Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

Plasmid

#71216

Purpose

dCas9 fused to human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A catalytic domain

Use

Lentiviral

Tags

dSpCas9-DNMT3A

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

Plasmid

#216387

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

Plasmid

#85453

Purpose

Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

Depositor

Aviv Regev

Article

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-sgRNA-Backbone-UP-TdTomato

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

deletion of AA123-365 in tdTomato (please see dep…

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Expression

Bacterial

Promoter

PlacIq and Ptaq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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