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pTLR-donor

Plasmid

#118999

Purpose

Repair template for the TLR reporter

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PB-PGK-teD-Cit-hcvs-DHFR

Plasmid

#116050

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

teD-Cit-hcvs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pOX-twk18

Plasmid

#16430

Depositor

Larry Salkoff

Article

Kunkel et al J Neurosci. 2000 Oct 15. 20(20):7517-24.

Insert

Tandem pore domain K+ channel twk-18 (twk-18 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
PB-PGK-tvD-Cit-tevs-DHFR

Plasmid

#116053

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
pGrpE-Wasabi

Plasmid

#86111

Purpose

mWasabi reporter gene driven by GrpE promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pGrpE

Available Since

Feb. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKB253

Plasmid

#170036

Purpose

Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
K5L7

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

May 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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NK590

Plasmid

#109084

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mPapaya

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]

Plasmid

#107852

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348V in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSGE_91_4xUAS_dCP

Plasmid

#71169

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

91_4xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]

Plasmid

#107851

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309F in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S]

Plasmid

#107850

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348S in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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NK594

Plasmid

#109085

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

TagRFP-T

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1330 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

Plasmid

#107839

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

Wildtype in splicing reporter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK649

Plasmid

#109105

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTU-B-005

Plasmid

#124413

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-005

Plasmid

#124414

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_32

Plasmid

#109236

Purpose

PUPsp2 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp2

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pOX-twk29

Plasmid

#16124

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only