Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,082 results for: cloning

Clear keywords
Showing: 11461 - 11480 of 63082 results

  1. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    63196-plasmid-map-sequence-id-104896

    Enlarge

  2. pAV113

    Plasmid
    #63180
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63180-plasmid-map-sequence-id-104851

    Enlarge

  3. pMuLE ENTR 7SK-miR-30 L1-L4

    Plasmid
    #62180
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid
    #62127
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62127-plasmid-map-sequence-id-112700

    Enlarge

  5. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid
    #62131
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62131-plasmid-map-sequence-id-112709

    Enlarge

  10. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid
    #176277
    Purpose
    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Martin Riccomagno
    Publication
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    MCS-P2A-mCherry
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    human Synapsin I
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176277-plasmid-map-sequence-id-350686

    Enlarge

  12. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

    Enlarge

  16. pHW2173

    Plasmid
    #255981
    Purpose
    N-terminal TAG-IN destination vector with SEC-LoxP for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD363 with LoxP)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255981-plasmid-map-sequence-id-512522

    Enlarge

  17. pHW2174

    Plasmid
    #255982
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox2272 for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255982-plasmid-map-sequence-id-511513

    Enlarge

  18. pHW2175

    Plasmid
    #255983
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox511I for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD364 with Lox511I)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255983-plasmid-map-sequence-id-511495

    Enlarge

Showing: 11461 - 11480 of 63082 results