We narrowed to 12,675 results for: nsf
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Plasmid#223158PurposeExpression of truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertMECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)
Plasmid#223152PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG002-ACE2(Mut)-HA
Plasmid#191570PurposeACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.DepositorInsertAngiotensin Converting Enzyme II (ACE2 Human)
UseLentiviral and RNAiTagsHA tagExpressionMammalianMutationCodon-optimized for human expression. Also contai…PromoterCMVAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS
Plasmid#209787PurposeExpresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)DepositorInsertTNT4, TadA8e, SpRY N, inteinN, miR122 TS
UseAAVPromoterTNT4Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgFAAH
Plasmid#209197PurposeMutagenesis of FaahDepositorAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1-CD20-Puro
Plasmid#209755PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V2-CD20-Puro
Plasmid#209756PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
1529_pAAV-CB-FKBP12-BirA-HA
Plasmid#208200PurposeExpress the murine FKBP12-BirA-HA fusion protein in AAV vectorDepositorAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK
Plasmid#202541PurposeAn adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoterDepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVss-U6-sgHTT51-7sk-Cas9
Plasmid#190901PurposeAAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNADepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1
Plasmid#190902PurposeAAV vector expressing thew GFP reporter gene and human sgHTTDepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shAtxn1
Plasmid#180387PurposeProducing AAV that encodes mouse Atxn1 shRNA with miR-E backboneDepositorAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113701PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113699PurposeDox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795AAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCH1:HDT1,HDT2-RNAi
Plasmid#108446PurposeArabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoterDepositorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC3
Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
Tags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV2_hSyn_Aurora_CA_Citrine
Plasmid#98219PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Aurora_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationV59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…Promoterhuman synapsinAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only