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pBEL1806

Plasmid

#139910

Purpose

Expression plasmid for LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L243N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1862

Plasmid

#139909

Purpose

Complementation plasmid for LetAB with LetB mutant L359N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L359N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1861

Plasmid

#139908

Purpose

Complementation plasmid for LetAB with LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L358N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1860

Plasmid

#139907

Purpose

Complementation plasmid for LetAB with LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L355N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1857

Plasmid

#139904

Purpose

Complementation plasmid for LetAB with LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L243N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2-pA-L4

Plasmid

#141062

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pExo84-CRU 303

Plasmid

#131165

Purpose

For C-terminal genomic integration of Cub-R-URA3, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Insert

ScExo84

Tags

CRU

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC26

Plasmid

#104691

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC26 (AT1G53025 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB325

Plasmid

#113669

Purpose

expresses Ulp1_R1 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R1

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtaC

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggAag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->gCtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW213

Plasmid

#113235

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->Cgtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtTg

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

Plasmid

#107855

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G368W and W385G in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

Plasmid

#90036

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-LEFT

Use

TALEN

Tags

3x Flag and Fok1-DD domain

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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