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HKDC1 gRNA (BRDN0001148616)

Plasmid

#76827

Purpose

3rd generation lentiviral gRNA plasmid targeting human HKDC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HKDC1 (HKDC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HKDC1 gRNA (BRDN0001146218)

Plasmid

#76828

Purpose

3rd generation lentiviral gRNA plasmid targeting human HKDC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HKDC1 (HKDC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HKDC1 gRNA (BRDN0001145441)

Plasmid

#76829

Purpose

3rd generation lentiviral gRNA plasmid targeting human HKDC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HKDC1 (HKDC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HKDC1 gRNA (BRDN0001148023)

Plasmid

#76830

Purpose

3rd generation lentiviral gRNA plasmid targeting human HKDC1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HKDC1 (HKDC1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CA gRNA (BRDN0001146906)

Plasmid

#77417

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GRK5 gRNA (BRDN0001148840)

Plasmid

#76274

Purpose

3rd generation lentiviral gRNA plasmid targeting human GRK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GRK5 (GRK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRDT gRNA (BRDN0001146712)

Plasmid

#77875

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRDT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRDT (BRDT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001144738)

Plasmid

#77876

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001145485)

Plasmid

#77877

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGFR4 gRNA (BRDN0001147333)

Plasmid

#77570

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR4 (FGFR4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGFR4 gRNA (BRDN0001146151)

Plasmid

#77571

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR4 (FGFR4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGFR4 gRNA (BRDN0001148634)

Plasmid

#77572

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR4 (FGFR4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HUNK gRNA (BRDN0001148239)

Plasmid

#75940

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-hETFDH-WT

Plasmid

#249046

Purpose

Expression of human wildtype ETFDH

Depositor

Ivan Topisirovic

Article

Papadopoli et al eLife, 2025 [Reviewed Preprint] 14:RP106

Insert

ETFDH (ETFDH Human)

Use

Lentiviral

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-WPRE-bGHpA

Plasmid

#231018

Purpose

For strong lentivirus-mediated expression of GFP

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Enhanced GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-dTomato-WPRE-bGHpA

Plasmid

#231019

Purpose

For strong lentivirus-mediated expression of dTomato

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

dTomato

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-W174A-EGFP

Plasmid

#179933

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing W174A mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

W174A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-F44E-EGFP

Plasmid

#179936

Purpose

C terminal fusion of EGFP to mouse HP1α (Cbx5) containing F44E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F44E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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