We narrowed to 17,896 results for: emb

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HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

Plasmid

#217683

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRB3 (BAI3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-856

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-Nir1-LNS2

Plasmid

#220084

Purpose

high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

mCherry-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)

Plasmid

#242572

Purpose

Gateway 3' entry clone containing an IRES Luciferase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-Luciferase-WPRE-bGH-pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway subcloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pME TAEL2.0-5xC120-minpro (JDW 1109)

Plasmid

#224507

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL2.0-5xC120

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

Plasmid

#224500

Purpose

Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA-pA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-dr-myl7-pro (JDW 1148)

Plasmid

#229836

Purpose

A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

cmlc2/myl7

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

Plasmid

#242589

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh (actin nanobody)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-LMNB1

Plasmid

#207770

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-RIGI-Apex- T2A-EGFP

Plasmid

#167288

Purpose

Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

APEX2 and EGFP

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ A-MFN2-Strep (SB253)

Plasmid

#227602

Purpose

Inducible expression of Strep-tagged MFN2 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

MFN2 (MFN2 Human)

Tags

Twin-StrepTag, 10xHis

Expression

Yeast

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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ACBE-Q

Plasmid

#204608

Purpose

Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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PATagRFP-GRP78

Plasmid

#238419

Purpose

Expresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

PATagRFP

Expression

Mammalian

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-CAAX

Plasmid

#208683

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-CENPA

Plasmid

#227283

Purpose

Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfrbeta-EGFPN

Plasmid

#66790

Purpose

Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRbeta (Pdgfrb Mouse)

Tags

C-term EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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