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pAc5.1B-EGFP-DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147846

Purpose

Insect Expression of DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1710-2505_J

Plasmid

#146701

Purpose

Insect Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1710-2505 (Not1 Fly)

Expression

Insect

Mutation

one non silent mutation (A1747T) and one silent m…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)-MutTER

Plasmid

#178035

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted and Changed T(8…

Promoter

T7LacI

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-HsGIGYF2_1-718_AI

Plasmid

#148960

Purpose

Mammalian Expression of HsGIGYF2_1-718

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-718_AI

Plasmid

#148948

Purpose

Mammalian Expression of HsGIGYF2_1-718

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI

Plasmid

#148950

Purpose

Mammalian Expression of HsGIGYF2_1-718-YFWFtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_280-310_AI

Plasmid

#148951

Purpose

Mammalian Expression of HsGIGYF2_280-310

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_280-310 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI

Plasmid

#148952

Purpose

Mammalian Expression of HsGIGYF2-F300AF306A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2-F300AF306A (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2-W288A_AI

Plasmid

#148953

Purpose

Mammalian Expression of HsGIGYF2-W288A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2-W288A (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI

Plasmid

#148954

Purpose

Mammalian Expression of HsGIGYF2-W288AF300AF306A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2-W288AF300AF306A (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF

Plasmid

#148691

Purpose

Mammalian Expression of HsGIGYF2-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2-YYMLtoAAAA (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

Plasmid

#146702

Purpose

Insect Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1710-2505 (Not1 Fly)

Expression

Insect

Mutation

two non silent mutations (A1747T, G1999S) and one…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF

Plasmid

#148686

Purpose

Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147860

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W

Plasmid

#147845

Purpose

Insect Expression of DmNot1_812-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)

Plasmid

#178034

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted

Promoter

T7LacI

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV mCgrrf1-V5

Plasmid

#175128

Purpose

Lentiviral expression of mouse Cgrrf1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cgrrf1 (Cgrrf1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_EVDm1

Plasmid

#167121

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

mutated four nucleotides at the snoRNA U1 binding…

Promoter

CaMV 35S

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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