We narrowed to 68,126 results for: Cel

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCG

Plasmid

#164563

Purpose

GFP

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-GgVCL/*TBM

Plasmid

#162790

Purpose

MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Mutated Gallus gallus vinculin (G454V) (VCL Chicken)

Tags

IRES-GFP

Expression

Bacterial and Mammalian

Mutation

changed Gly 454 to Val

Promoter

beta actin

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir374b~421-CMV2-mir1

Plasmid

#164674

Purpose

Express miR-374b~421 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-374b~421 (MIR374B Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir374b-CMV2-mir1

Plasmid

#164672

Purpose

Express miR-374b and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-374b (MIR374B Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir181b-1~125-CMV2-mir1

Plasmid

#164671

Purpose

Express miR-181b-1~125a and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-181b-1~125a (MIR181B1 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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CTMP

Plasmid

#129560

Purpose

cytoplasmic marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

mCherry

Expression

Bacterial

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK_1

Plasmid

#163462

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 1 targeting NADK (NADK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-HisFlag-USP19ER

Plasmid

#155244

Purpose

for generating adenoviruses expressing the ER isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Mutation

H118R- please see dep. comments

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-mChe

Plasmid

#153423

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-BIOT

Plasmid

#140186

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Biotinylation and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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D57_Ld_190640

Plasmid

#135870

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

extracellular receptor, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD

Plasmid

#120710

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-TAF12-HFD(50-130)

Plasmid

#105615

Purpose

transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hsp70:EB3-mKate2

Plasmid

#105965

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pME GFP-DCX

Plasmid

#105962

Purpose

entry clone for gateway cloning

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NP_835365.1 (DCX Human)

Tags

6xHis-thrombin cleavage site and EGFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPN158

Plasmid

#91687

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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