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pYO1101

Plasmid

#235708

Purpose

Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
ATG14L (ATG14 Human)

Expression

Mammalian

Promoter

CAG and CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73

Plasmid

#231751

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73deltaKIM

Plasmid

#231752

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73-YY/AA

Plasmid

#231753

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaNTD(700-1490)

Plasmid

#231734

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12-KiD(700-1082)

Plasmid

#231736

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaRS(delta104-380)

Plasmid

#231737

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

Plasmid

#231739

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

Plasmid

#231740

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A

Plasmid

#223560

Purpose

Alias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc

Use

Lentiviral

Expression

Mammalian

Mutation

W139A mutation in GINIP sequence

Promoter

CMV

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET14-H2Kb-ST3

Plasmid

#223221

Purpose

Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)

Tags

Spytag

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2374

Plasmid

#225631

Purpose

High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple391 (DRD2 Human)

Use

Unspecified

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2368

Plasmid

#225632

Purpose

High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple385 (GPR6 Human)

Use

Unspecified

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2156

Plasmid

#158099

Purpose

High Copy number plasmid containing SYN1 insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

SYN1 (SYN1 Human)

Promoter

SYN1

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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(attB) tdMCP-tagged BRaf

Plasmid

#226165

Purpose

Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-BRaf (BRAF Human)

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2372

Plasmid

#225630

Purpose

High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple389 (ADORA2A Human)

Use

Unspecified

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC4 CD

Plasmid

#224251

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC7 CD

Plasmid

#224341

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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