Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,162 results for: NAK

Showing: 1141 - 1160 of 3162 results

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mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173865

Purpose

Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaCoiledCoil-LIN-41

Plasmid

#52720

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 328–447

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-C12AC15A-LIN-41

Plasmid

#52725

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12 and 15 to alanines

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaBBox-LIN-41

Plasmid

#52719

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 194-320

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaFilamin-LIN-41

Plasmid

#52721

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 483–583

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T-2 GST-mouse Fis1 (ΔTM domain)

Plasmid

#252268

Purpose

Expression of GST-mouse Fis1 (ΔTM domain) in bacteria

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (deltaTM domain) (Fis1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

moue Fis1 1-121 amino acids

Promoter

tac

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (L110A)

Plasmid

#252265

Purpose

Retroviral expression of EGFP-mouse Fis1 (L110A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (L110A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating leucine 110 to alanine (L110A) in Fis1 r…

Promoter

CMV

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (W40A)

Plasmid

#252263

Purpose

Retroviral expression of EGFP-mouse Fis1 (W40A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (W40A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating tryptophan 40 to alanine (W40A) in Fis1 …

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (V79A)

Plasmid

#252264

Purpose

Retroviral expression of EGFP-mouse Fis1 (V79A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (V79A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating valine 79 to alanine (V79A) in Fis1 resu…

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1616A/S1691A

Plasmid

#215909

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616A, S1691A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D

Plasmid

#215912

Purpose

Expression of full-length TNRC6A with S1332D/S1346D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1332D, S1346D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1332A/S1346A

Plasmid

#215913

Purpose

Expression of full-length TNRC6A with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D

Plasmid

#215914

Purpose

Expression of full-length TNRC6A with S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1616D, S1691D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A

Plasmid

#215915

Purpose

Expression of full-length TNRC6A with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1616A, S1691A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A

Plasmid

#215902

Purpose

Expression of TNRC6A-CIM1 domain with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A/S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A

Plasmid

#215903

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A

Plasmid

#215904

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616A, S1691A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D

Plasmid

#215906

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332D, S1346D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A

Plasmid

#215907

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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