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pFH33

Plasmid

#125885

Purpose

Level0 Wheat TaU3 Pol III promoter with transcription Start-A

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Wheat TaU3 Pol III promoter with transcription Start-A

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMIN-0

Plasmid

#197782

Purpose

Level 2 destination vector: minimal (non-T-DNA) backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRN104

Plasmid

#127231

Purpose

35S::AFR5::35S terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Mpdelta

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-4

Plasmid

#197814

Purpose

Level 0 J1-J4 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-11

Plasmid

#197815

Purpose

Level 0 J1-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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GGE077

Plasmid

#120779

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_RedStarII)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

G1_RedStarII

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFH37

Plasmid

#126011

Purpose

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Promoter

T7 sense, T3 antisense

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pCON1-ProA.mCherry

Plasmid

#112519

Purpose

ProA driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA mCherry TT

Expression

Bacterial

Promoter

ProA

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Expression

Bacterial

Mutation

C114S, L118S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pCON1-ProC.mCherry

Plasmid

#112521

Purpose

ProC driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC mCherry TT

Expression

Bacterial

Promoter

ProC

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCON1-ProA.gfp

Plasmid

#112515

Purpose

ProA driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA gfpmut3 TT

Expression

Bacterial

Promoter

ProA

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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UbcH10

Plasmid

#8504

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Expression

Bacterial

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
PL06010B1H03 (smedwi1)

Plasmid

#35699

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi1

Promoter

T7 sense, T3 antisense

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Tags

NA

Expression

Yeast

Promoter

NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFH39

Plasmid

#126013

Purpose

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge