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pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

Plasmid

#182344

Purpose

sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Spacer 4

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

Plasmid

#182344

Purpose

sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Spacer 4

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine

Plasmid

#197680

Purpose

"Insert(high)-pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine

Plasmid

#197680

Purpose

"Insert(high)-pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine

Plasmid

#197682

Purpose

"Insert(low)-pEF1s(low)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine

Plasmid

#197682

Purpose

"Insert(low)-pEF1s(low)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

Plasmid

#197681

Purpose

"Insert(high)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

Plasmid

#197681

Purpose

"Insert(high)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

Plasmid

#91820

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

Plasmid

#91820

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

Plasmid

#180806

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y243F, Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

Plasmid

#180806

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y243F, Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

Plasmid

#180807

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y226F, Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

Plasmid

#180807

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y226F, Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

Plasmid

#180808

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y226F, Y243F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

Plasmid

#180808

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y226F, Y243F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge