We narrowed to 23,671 results for: CRISPR

1. pAT9675-CBE

   Plasmid

   #163007

   Purpose

   plasmid expressing CBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   CBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP Y93H TC editing reporter

   Plasmid

   #198882

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Y93H

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-sgTel

   Plasmid

   #122659

   Purpose

   Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-NEAT1pr_v1

   Plasmid

   #97081

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v1
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-E1347A-nCas9(D10A)-UGI

   Plasmid

   #187410

   Purpose

   DddA E1347A variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA E1347A variant
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_124

   Plasmid

   #133461

   Purpose

   EF1a promoter expresses SpyoCas9 and P2A provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   SpyoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS12: pDonor(AvCAST)

   Plasmid

   #168145

   Purpose

   AvCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST right end (RE)
   AvCAST left end (LE)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-cGAS g2 Cas9-T2A-mCherry

   Plasmid

   #164253

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVV205

    Plasmid

    #215342

    Purpose

    Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23105

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-AAAAA-nCas9(D10A)-UGI

    Plasmid

    #187409

    Purpose

    DddA AAAAA variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA AAAAA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF221

    Plasmid

    #121669

    Purpose

    U6-sgRNA EF1a-mCherry

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6630

    Plasmid

    #106160

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-NEAT1pr_v2

    Plasmid

    #97082

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1pr_v2
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFD3-frame_selector_2

    Plasmid

    #127555

    Purpose

    Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    Alejandro Chavez, George Church

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS13: pTarget(AvCAST)

    Plasmid

    #168146

    Purpose

    Target plasmid containing AvPSP1 and attachement site for AvCAST.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PAM for AvCAST + AvPSP1
    glmS
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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