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pMDC32-FIT2

Plasmid

#96990

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Promoter

35S

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem194 pEGFP-N2 (1074)

Plasmid

#62027

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Tmem194 pmRFP-N2 (1389)

Plasmid

#62048

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2415

Plasmid

#36816

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT1-TALEN_Right (Ext1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
TAL2204

Plasmid

#36667

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT2-TALEN_Left (Ext2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2205

Plasmid

#36668

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT2-TALEN_Right (Ext2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2228

Plasmid

#36683

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SDHC-TALEN_Left (SDHC Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2229

Plasmid

#36684

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SDHC-TALEN_Right (SDHC Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
Sus1/PET32a

Plasmid

#26963

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sus1 (SUS1 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBS FAM20C

Plasmid

#19214

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

FAM20C (FAM20C Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pSP64T Activin betaB (DM#108)

Plasmid

#15045

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

Insert

Activin Beta b (inhbb Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pPS1070

Plasmid

#1355

Depositor

Pamela Silver

Article

Seedorf et al Proc Natl Acad Sci U S A 1997 Aug 5;94(1

Insert

KAP123 (KAP123 Budding Yeast)

Tags

sGFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pEF-mRuby3

Plasmid

#179730

Purpose

Mammalian expression of mRuby3 from the EF1 promoter

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

mRuby3

Expression

Mammalian

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-kcnk18

Plasmid

#209000

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk18 (kcnk18 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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