We narrowed to 25,057 results for: Nes

1. pIS1 Cyclin D2 long UTR

   Plasmid

   #21645

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 long UTR (CCND2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

   Plasmid

   #21800

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   PSMD14 (Psmd14 Mouse)
   

   Use

   Tags

   GST and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-PM-CaNARi

   Plasmid

   #64850

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNARi
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. enAsCas12a-mSA

   Plasmid

   #182954

   Purpose

   Express enAsCas12a-mSA

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   enAsCas12a-mSA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-attBr-Cre-rc-attP

   Plasmid

   #51264

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-Cre-rc-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVenus-Sec16A

   Plasmid

   #36157

   Purpose

   Expresses Sec16A as a Venus FP fusion

   Depositor

   David Stephens

   Article

   Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

   Insert

   Sec16A (SEC16A Human)
   

   Use

   Tags

   Venus (EYFP derivative)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHSP70B-CP6-TK

   Plasmid

   #165881

   Purpose

   Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Basic Selection Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-mFzd10-1D4

   Plasmid

   #42272

   Depositor

   Chris Garcia, Jeremy Nathans

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 10 (Fzd10 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pFA-LEU2-I-SceI

    Plasmid

    #67595

    Purpose

    I-SceI expression regulated by tetracycline inducible system in S. pombe

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    nmt terminator
    I-SceI
    TetR
    

    Use

    Gene targeting in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNHA-C1-HsUPF2-siRNAres_I

    Plasmid

    #146567

    Purpose

    Mammalian Expression of HsUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsUPF2-siRNAres (UPF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSicoR mouse Dicer3

    Plasmid

    #14781

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (Dicer1 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-puro-CLIP-FLAG-CD59

    Plasmid

    #50375

    Purpose

    Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    CLIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.11 promPDE4D3 Ebox mut

    Plasmid

    #27304

    Depositor

    David E. Fisher

    Article

    Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

    Insert

    prom PDE4D3 (PDE4D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Ebox CACATG changed to GAGATG

    Promoter

    Available Since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMX-PGC1

    Plasmid

    #6

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2005

    Availability

    Academic Institutions and Nonprofits only

20. GFP-mROCK1-delta4

    Plasmid

    #101292

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    76-1354 aa

    Promoter

    CMV

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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