We narrowed to 23,671 results for: CRISPR

1. pCMV_ABEmaxNG‐NGA

   Plasmid

   #187055

   Purpose

   ABEmax-SpCas9-NGA

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   ABEmaxNG‐NGA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6638

   Plasmid

   #106165

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

   Plasmid

   #84249

   Purpose

   Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgSV40
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSimpleII-NmCas9-FLAG

   Plasmid

   #47872

   Purpose

   FLAG tagged NmCas9 with no NLS.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET47b-T7-dAsCas12f

   Plasmid

   #204641

   Purpose

   Expression of an inactive AsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   inactive AsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D225A

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIO-LbCpf1

   Plasmid

   #137847

   Purpose

   LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   LbCpf1-NLS-FLAG
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCD017

   Plasmid

   #177322

   Purpose

   dCas9 expression

   Depositor

   James Carothers

   Article

   Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

   Insert

   dCas9
   

   Use

   CRISPR

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH113

   Plasmid

   #128210

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEC581

    Plasmid

    #174369

    Purpose

    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    RFP with PAM rich sequence upstream promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23117

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-BE4Gam

    Plasmid

    #138269

    Purpose

    Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BE4Gam
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORE303N_CEN12RRFuzzy3MYB

    Plasmid

    #194441

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA array targeting CEN1, RR, Fuzzy3MYB
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    MtU6

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKE014

    Plasmid

    #207908

    Purpose

    GG entry for zCas9i expression

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    SpCas9_introns_2xNLS-Pea3A(T) term
    

    Use

    CRISPR

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH49

    Plasmid

    #128211

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Rice codon optimized

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3D-Cas9n-UGI

    Plasmid

    #119137

    Purpose

    Base editor made from APOBEC3D

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3D-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB6631

    Plasmid

    #106161

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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