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Str-KDEL_SBP-mCherry-LAMP1

Plasmid

#222318

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP L273D-L276D

Plasmid

#114182

Purpose

ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP L273D-L276D (ORAI1 Human)

Tags

YFP

Expression

Mammalian

Mutation

L273D/L276D

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PHF19

Plasmid

#68864

Purpose

GW entry vector for PHF19

Depositor

Adrian Bracken

Article

Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

Insert

PHF19 (PHF19 Human)

Use

Gateway entry vector

Promoter

none

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
FKBP-LBR(1-245)-GFP

Plasmid

#222425

Purpose

To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(1-245aa) (LBR Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncation (Includes aa 1-245)

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHAND1-T2A-dTom-PGK-Neo

Plasmid

#209838

Purpose

Tomato reporter plasmid targeting the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

Insert

HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)

Use

Human targeting

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h1mut

Plasmid

#86690

Purpose

For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)

Tags

HA

Mutation

mutated region NHDC (asparagine 273 to cysteine 2…

Promoter

T3

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag

Plasmid

#124915

Purpose

For stable expression sgRNA resistant Flag tagging mLST8_

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)

Expression

Mammalian

Mutation

G245S

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMPx3ALFA

Plasmid

#225136

Purpose

R2pH-LAMP1-labelled lysosomes with 3xLFA

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)

Expression

Mammalian

Mutation

p53 A138V

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-mKdm2b (del CxxC domain)-Flag

Plasmid

#61740

Purpose

Express CxxC domain deleted mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Flag

Mutation

delete aa 588-624

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
qTAG-N-Puro-moxGFP-TUBA1B

Plasmid

#207767

Purpose

Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1_mGFP-FGFR3-K508M

Plasmid

#191753

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1523A>T (p.K508M) + silent mutation c.1515A&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+-PIF6-EGFP

Plasmid

#154913

Purpose

1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)

Use

Xenopus/avian/zebrafish

Tags

EGFP

Expression

Mammalian

Mutation

1-100 amino acids of Arabidopsis PIF6

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28-Strep-OPA1(253-960) (SB263)

Plasmid

#227612

Purpose

Inducible expression of Strep-tagged Opa1(253-960) in E.coli

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Bacterial

Mutation

contains aa 253-960 only

Promoter

T7

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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