We narrowed to 13,357 results for: BASE

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pHyg-CUbo-yeGFP-His6

Plasmid

#212782

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo-9myc

Plasmid

#212786

Purpose

C-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K63R)-9myc

Plasmid

#212788

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-9myc

Plasmid

#212789

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo-AID*-GFP

Plasmid

#212770

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K63R)

Plasmid

#212764

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K48R)

Plasmid

#212763

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLINE1mORF1-ΔORF2-scFvFc

Plasmid

#206023

Purpose

The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pPhiC31-actIIORF4

Plasmid

#190785

Purpose

The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

T5 terminator
actII-4
kasOp*

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-CKAR

Plasmid

#123345

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

Plasmid

#123335

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HxHLICNot

Plasmid

#140183

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

12xHis

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-BIOT

Plasmid

#140186

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Biotinylation and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRcCMV-STAT31F

Plasmid

#134725

Purpose

Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707

Depositor

Jim Darnell, Curt Horvath

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

STAT3-STAT1 Fusion (STAT3, STAT1 Human)

Tags

FLAG and human STAT3 aa 1-514 joined to human STA…

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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