We narrowed to 1,917 results for: FRT

1. pSEVA12DKR-J

   Plasmid

   #58306

   Purpose

   GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA12DKR-A

   Plasmid

   #58304

   Purpose

   GeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA12DKR-E

   Plasmid

   #58305

   Purpose

   GeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA12TEC-B

   Plasmid

   #58307

   Purpose

   GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAFNF-GFP

   Plasmid

   #13772

   Purpose

   Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   EGFP
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAFNF-DsRed

   Plasmid

   #13771

   Purpose

   Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   DsRed2
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FR_luc

   Plasmid

   #31098

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   firefly luciferase
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pBALU1

   Plasmid

   #69316

   Purpose

   Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp

   Depositor

   Oliver Hobert

   Article

   Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

   Insert

   GFPint-FgF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBALU6

    Plasmid

    #69321

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    Venus-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFLLFLN

    Plasmid

    #40030

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    FRT-Lox5171-Lox2272-FRT-loxP
    Neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    None and SV40

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBALU4

    Plasmid

    #69319

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of mChO (mCherry variant)

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    mChOint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pBALU3

    Plasmid

    #69318

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    CFPint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBALU2

    Plasmid

    #69317

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    YFPint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pKHR8

    Plasmid

    #74625

    Purpose

    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Inserts

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-crystallin and cmlc2

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ciCas9_pcDNA5

    Plasmid

    #100550

    Purpose

    Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fret-Stop-Fret TOPO

    Plasmid

    #22774

    Depositor

    Tyler Jacks

    Article

    Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

    Insert

    fret-STOP-fret
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYC44

    Plasmid

    #63903

    Purpose

    Initial Integrative Vector

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FRT::3'UTRcta::NAT::FRT

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBALU9

    Plasmid

    #69324

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    gSL2-NLS-GFPint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pNM3746

    Plasmid

    #154109

    Purpose

    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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