We narrowed to 2,424 results for: MEA;

1. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

   Plasmid

   #187320

   Purpose

   dCas9 expression vector with cloning site for C terminal fusions

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 AIF-mScarlet

   Plasmid

   #129576

   Purpose

   Expression of mScarlet fused AIF

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   AIF (AIFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 AIF-mClover

   Plasmid

   #129575

   Purpose

   Expression of mClover fused AIF

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   AIF (AIFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

   Plasmid

   #192200

   Purpose

   Retro-BCMACAR-PRODH2

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

   Plasmid

   #202059

   Purpose

   Inducible AAV activator

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
   

   Use

   AAV; Aav with reporter

   Tags

   Expression

   Mammalian

   Mutation

   ITRs intact

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMK ENTR 4 CMV GST mTagBFP

   Plasmid

   #206259

   Purpose

   ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   GTS mTagBFP
   

   Use

   Multimate/gateway entr 4

   Tags

   BFGT1 (Golgi localisation peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAuroraA GFP-AURKA-GFP

   Plasmid

   #157765

   Purpose

   Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   promoter CMV replaced by AurkA promoter

   Promoter

   AURKA minimal

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX_hDDX5_FL_D248N

   Plasmid

   #88872

   Purpose

   inducible expression of GST-DDX5 D248N in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D248N

   Promoter

   Tac

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

   Plasmid

   #195543

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCerulean3-Bcl-W-FUGW

    Plasmid

    #166751

    Purpose

    Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Bcl-W (Bcl2l2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SITS-MmCav1-EGFP-APEX2

    Plasmid

    #170525

    Purpose

    Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Caveolin1 (Cav1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    APEX2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-Nix-delta TM

    Plasmid

    #100755

    Purpose

    Mammalian expression of Nix dominant negative

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Nix-_TM (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    TM deletion

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBGE3.0

    Plasmid

    #165988

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNL3.1-Erg

    Plasmid

    #182706

    Purpose

    Express Erg enhancer in mammalian cells

    Depositor

    Robert Rowe

    Article

    Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

    Insert

    Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus-Bik-4E-pEGFP-C1

    Plasmid

    #166744

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Bik-4E (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Human, Synthetic)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myc-Nix-MaoB

    Plasmid

    #100757

    Purpose

    Mammalian expression of mitochondria targetted Nix

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Nix-MaoB (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    TM domain replaced by MaoB

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. EphA2 430-976 pcDNA3.1

    Plasmid

    #102738

    Purpose

    Expression of c terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (430-976aa) (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV9-hSyn iCyt_BrEt_SnFR_PM

    Plasmid

    #177757

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn iCyt_BrEt_SnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGE2.0

    Plasmid

    #165984

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge