We narrowed to 2,869 results for: human cdnas

1. pCDNA3.1-5'dGFP3'

   Plasmid

   #26425

   Depositor

   Erin Schuman

   Article

   Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

   Insert

   5'dGFP3'
   

   Use

   Tags

   CamKIIa 3'-UTR, CamKIIa 5'-UTR, and dGF…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

2. HA GSK3 beta S9A pcDNA3

   Plasmid

   #14754

   Purpose

   Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

   Depositor

   Jim Woodgett

   Article

   Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701

   Insert

   GSK3 beta S9A (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A. Constitutively active mutant.

   Promoter

   Available since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hAsCpf1(TATV) (pY220)

   Plasmid

   #89353

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TATV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Golgi-ExRai-AktAR2

   Plasmid

   #184051

   Purpose

   Golgi-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Golgi-ExRai-AktAR2
   

   Use

   Tags

   N-terminal 35 amino acids from human endothelial …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT TO myc hSeparase

   Plasmid

   #59820

   Purpose

   Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nano-lantern-H2B/pcDNA3

   Plasmid

   #51971

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 NKX6-2-Venus R200W

   Plasmid

   #209179

   Purpose

   Mammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent protein

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (R200W) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (R200W)

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 NKX6-2-Venus L163V

    Plasmid

    #209178

    Purpose

    Mammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (L163V) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (L163V)

    Promoter

    CMV

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1-TSF-tepsin

    Plasmid

    #196636

    Purpose

    Expresses the two Strep/one FLAG (TSF)-tagged human tepsin in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Tags

    two Strep/one FLAG (TSF)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-RNMT 1-120

    Plasmid

    #112706

    Purpose

    Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 121-476

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

    Plasmid

    #52006

    Purpose

    Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    5utr-MAVS-ORF1mut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation removing the start codon for ORF1

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid

    #50914

    Purpose

    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C N-term deletion of aa13-222 in hNKCC1…

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid

    #50865

    Purpose

    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3/Flag-WTAP

    Plasmid

    #53741

    Purpose

    For expression of WTAP (Wilms tumor 1 associated protein) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Wilms tumor 1 associated protein (WTAP Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5FRT/TO-Npl4-Strep-HA

    Plasmid

    #113495

    Purpose

    Expression of human Npl4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Npl4 (NPLOC4 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/TO-Ufd1-Strep-HA

    Plasmid

    #113474

    Purpose

    Expression of human Ufd1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    Ufd1 (UFD1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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