Addgene

We narrowed to 4,932 results for: puc

Showing: 1161 - 1180 of 4932 results

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL658

Plasmid

#167837

Purpose

Encodes OptoLAC1B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL659

Plasmid

#167838

Purpose

Encodes OptoLAC2B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-dSa-VP64

Plasmid

#99680

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x2

Plasmid

#63602

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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bbCas9pluspAAA

Plasmid

#82581

Purpose

The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

Depositor

Ivo Huijbers

Article

Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x4

Plasmid

#63598

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA17

Plasmid

#48990

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x7

Plasmid

#63601

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_FokI-1x4

Plasmid

#63604

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_FokI-1x7

Plasmid

#63607

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_FokI-1x6

Plasmid

#63606

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_FokI-1x3

Plasmid

#63603

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pJUMP38-1A(sfGFP)

Plasmid

#126984

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_FokI-1x5

Plasmid

#63605

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-6XDSR

Plasmid

#49865

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-3XDSR

Plasmid

#49863

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge