We narrowed to 7,144 results for: val

1. pET28a-Hook3 I136A, I139A, M142A

   Plasmid

   #74617

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS424-TUB2 - human TUBB6 Cterminal tail

   Plasmid

   #60403

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pK-eCFP

   Plasmid

   #176559

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169316

   Purpose

   Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SIN40C.SFFV.mTagBFP2.miR-ctrl

   Plasmid

   #169312

   Purpose

   Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJL1

   Plasmid

   #117429

   Purpose

   Expression of ARL1

   Depositor

   Richard Kahn

   Article

   Van Valkenburgh et al J Biol Chem. 2001 Jun 22;276(25):22826-3

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Hook3 Q147A, M151A, I154A

   Plasmid

   #74616

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Q147A, M151A, I154A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Hook3 D102A, E108A

   Plasmid

   #74618

   Purpose

   Expresses a double mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS424-TUB2 - human TUBB1 Cterminal tail

    Plasmid

    #60400

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRS424-TUB2 - human TUBB3 Cterminal tail

    Plasmid

    #60397

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C127S; TUB2 amino acids 429 - end, replaced with …

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRS424-TUB2 - human TUBB7 Cterminal tail

    Plasmid

    #60399

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRS424-TUB2 - human TUBB8 Cterminal tail

    Plasmid

    #60401

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRS424-TUB2 - human TUBB4a Cterminal tail

    Plasmid

    #60402

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pFastBac(His10-Lckg2a,Y394F,Y505F)

    Plasmid

    #177894

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Use

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A,Y394F,Y505F

    Promoter

    Polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a(cd96)

    Plasmid

    #177901

    Purpose

    Bacterial expression of his10-tagged human cd96 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD96 (aa 557-585 only) (CD96 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-hnRNPU-V5

    Plasmid

    #35974

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDual-eGFP(Y203)

    Plasmid

    #63559

    Purpose

    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Y203)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed threonine at position 203 with respect to…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HSC1-HS4-D4Z4c-GiP

    Plasmid

    #58541

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge