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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR

Plasmid

#61080

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

gRNA_hIRF1 promoter #12 (IRF1 Human)

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-A3A-seBE4max-IRES

Plasmid

#174698

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

A3A-seBE4max-IRES (APOBEC3A Human)

Tags

myc tag

Expression

Mammalian

Mutation

A3A-M13I, UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta6

Plasmid

#100883

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 489-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta7

Plasmid

#100884

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 668-781aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta5

Plasmid

#100882

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 400-666aa

Promoter

CMV

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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