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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,496 results for: grna

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Showing: 11821 - 11840 of 23496 results

  3. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  5. pCE27

    Plasmid
    #174405
    Purpose
    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174405-plasmid-map-sequence-id-349710

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  10. pGTRm

    Plasmid
    #127197
    Purpose
    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA PCR template
    Use
    Template plasmid for generation of golden gate pc…
    Promoter
    None
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127197-plasmid-map-sequence-id-248423

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  12. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  16. pCE41

    Plasmid
    #174433
    Purpose
    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174433-plasmid-map-sequence-id-349717

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  17. pU6T7

    Plasmid
    #71462
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)
    Depositor
    Willie Mark
    Article
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    human U6/phage T7
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    71462-plasmid-map-sequence-id-128762

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Showing: 11821 - 11840 of 23496 results