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pcDNA3-HA2-Cul4A-deltaROC

Plasmid

#20713

Depositor

Yue Xiong

Article

McCall et al Mol Cell Biol. 2008 Sep . 28(18):5621-33

Insert

Cullin 4A (CUL4A Human)

Tags

HA2

Expression

Mammalian

Mutation

Deletion of residues 594 to 612 from N-terminus

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex13

Plasmid

#35165

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-mAPPLE

Plasmid

#60696

Purpose

retroviral expression of rat Cx43 fused to mAPPLE

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

mAPPLE

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pTriEx4-ADCY6-C2 (917-1165 aa)

Plasmid

#113904

Purpose

His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Mutation

Please see Depositor Comments

Promoter

T7, CMV

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx4-ADCY6-C1(306-672 aa)

Plasmid

#113903

Purpose

His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Promoter

T7, CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST_CR2

Plasmid

#34923

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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LXSN-Cx43-S279D

Plasmid

#60707

Purpose

retroviral expression of rat Cx43 S279D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282A

Plasmid

#60709

Purpose

retroviral expression of rat Cx43 S282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282A

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282D

Plasmid

#60711

Purpose

retroviral expression of rat Cx43 S282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-YOD1-MutS2-C160S

Plasmid

#85668

Purpose

Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

C160S and I292Q and V295Q

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a del.SHD

Plasmid

#40036

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a del.SHD (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted SHD domain (1-143)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-B2UP49_GFP

Plasmid

#103071

Purpose

Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_2

Plasmid

#124911

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFP

Plasmid

#166676

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2AB

Plasmid

#40037

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2AB (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A and C2B domains (353-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2A

Plasmid

#40038

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A domain (353-487)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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nes374tk/lacZ

Plasmid

#47615

Purpose

used to create transgenic mice expressing LacZ reporter with Nestin enhancer

Depositor

Urban Lendahl

Article

Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.

Insert

nestin 2nd intron fragment (NES Human)

Use

Enhancer reporter

Tags

HSV tk promoter and lacZ

Mutation

374 bp from 2nd intron (comprising bp 1153 to 152…

Promoter

HSV tk promoter

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only