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pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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hPGK-ABE7.10-P2A-GFP

Plasmid

#162511

Purpose

Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

hPGK

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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EFS-ABE7.10-P2A-GFP

Plasmid

#162510

Purpose

Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pZA3

Plasmid

#158481

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA2

Plasmid

#158480

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA11

Plasmid

#158489

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA4

Plasmid

#158482

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA10

Plasmid

#158488

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA12

Plasmid

#158490

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJM143

Plasmid

#134166

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJM108

Plasmid

#134160

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL, E. coli codon optimized, CRISPR/Cas9 harden…

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJM107

Plasmid

#134159

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-GreBdm-D41N

Plasmid

#129691

Purpose

express E. coli GreB E82C/C68S/D41N

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GreB E82C C68S D41N (greB E. coli)

Tags

his6

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

Plasmid

#121825

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/p300core (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

Plasmid

#121826

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/Dnmt3a-3L (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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