We narrowed to 25,057 results for: Nes

1. pISH-Isl1

   Plasmid

   #84311

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Isl1. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   Isl1 (Isl1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

   Plasmid

   #174529

   Purpose

   Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Inserts

   PylS
   PylT
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOD_A0201

   Plasmid

   #91002

   Purpose

   Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   35S

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK1160

   Plasmid

   #179298

   Purpose

   Expresses dCas9 fused to P65

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-p65
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ad-shMKP-3

   Plasmid

   #131530

   Purpose

   Silence the MKP-3 expression

   Depositor

   Haiyan Xu

   Article

   Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

   Insert

   Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMB-TMEg

   Plasmid

   #61209

   Purpose

   Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtBCPsp-GFP-BCP
   

   Use

   Tags

   EGFP

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6HTC_IGF2BP2b-Halo

   Plasmid

   #175318

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP2 (IGF2BP2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Isoform B

   Promoter

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-Ub-VPS13D_dN

   Plasmid

   #176465

   Purpose

   express VPS13D lacking N-terminal 117 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Use

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Flag-YAP-del 50-59

   Plasmid

   #33065

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 50-59

   Promoter

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pHWBF06exp

    Plasmid

    #130831

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    SUN2
    

    Use

    Tags

    mCherry-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-SAC1C389S-FKBP

    Plasmid

    #108130

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1LC389S:FKBP(3-108) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQStrep2-TRAPPC3

    Plasmid

    #34711

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    trafficking protein particle complex 3 (TRAPPC3 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pH6HTC_CCDC86-Halo

    Plasmid

    #175323

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC86 (CCDC86 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3278 pSFFV-neo Bid mouse

    Plasmid

    #8765

    Depositor

    Stanley Korsmeyer

    Article

    Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

    Insert

    Bid ORF cDNA FL (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pHCKan-phoBp-GFPuv

    Plasmid

    #139073

    Purpose

    Low phosphate inducible promoter phoBp for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    phoBp-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RCAS (A) Hoxd13 (CT#441)

    Plasmid

    #13985

    Depositor

    Cliff Tabin

    Article

    Goff et al Development. 1997 Feb . 124(3):627-36.

    Insert

    Hoxd-13 (HOXD13 Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pCGN-ELK-1 R mut

    Plasmid

    #27159

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R box mutant: K249A/V250A/E251A

    Promoter

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pISH-Dlx6

    Plasmid

    #74345

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    distal-less homeobox 6 (Dlx6 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MALT1-DD (2G7R)

    Plasmid

    #25613

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MALT1-DD (MALT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains Death Domain

    Promoter

    Available Since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits