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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 62,357 results for: cloning

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Showing: 11861 - 11880 of 62357 results

  1. pIK198

    Plasmid
    #65629
    Purpose
    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Promoter
    U6
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65629-plasmid-map-sequence-id-111540

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  8. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3 constitutive promoter and native promoter
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  15. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid
    #133970
    Purpose
    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    mVenus
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available Since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133970-plasmid-map-sequence-id-267750

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Showing: 11861 - 11880 of 62357 results