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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pERB257

Plasmid

#67761

Purpose

Expresses KLC1-mCherry-eDHFR

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

KLC1-mCherry-eDHFR (Klc1 Rat)

Tags

eDHFR and mCherry

Expression

Mammalian

Mutation

contains Klc1 amino acids 1-175

Promoter

CAG

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEE495

Plasmid

#149291

Purpose

T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Ptn in situ probe

Plasmid

#45632

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Ptn in situ probe (Ptn Mouse)

Use

In situ

Mutation

fragment contains bp# 1241-1830 of NM_008973

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
KTK_001

Plasmid

#180517

Purpose

Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 P139A (#625)

Plasmid

#99945

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

P139A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo S100a10 in situ probe

Plasmid

#45603

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

s100a10 in situ probe (S100a10 Mouse)

Use

In situ

Mutation

fragment contains bp# 21-275 of BC025044

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 S137A (#627)

Plasmid

#99943

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S137A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Foxp2 in situ probe

Plasmid

#45620

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Foxp2 in situ probe (Foxp2 Mouse)

Use

In situ

Mutation

fragment contains bp#1709-2142 of AF339106

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3Am

Plasmid

#109364

Purpose

Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 S103L (#624)

Plasmid

#99942

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

S103L

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Cux2 in situ probe

Plasmid

#45623

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Cux2 in situ probe (Cux2 Mouse)

Use

In situ

Mutation

fragment contains nt 1069-1694 on NM_007804

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Nectin-3 in situ probe

Plasmid

#45633

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Nectin-3 in situ probe (Nectin3 Mouse)

Use

In situ

Mutation

fragment contains bp# 1626-2039 of NM_021495

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Crym in situ probe

Plasmid

#45601

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crym in situ probe (Crym Mouse)

Use

In situ

Mutation

fragment contains nt 756-1191 of NM_016669

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 C140S (#622)

Plasmid

#99947

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C140S

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1014

Plasmid

#239936

Purpose

Hsp18.2 (heat inducible) promoter driving the expression of sgRNA-B

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Hsp18.2::sgRNA-B

Use

Synthetic Biology

Expression

Plant

Mutation

N/A

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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LLP984

Plasmid

#239906

Purpose

TCTP-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-04 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP985

Plasmid

#239907

Purpose

TCTP-SynPro-05 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-05 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1020

Plasmid

#239942

Purpose

AtACT2 promoter driving the expression of dCas9-ZAT10(2x)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

AtACT2::dCas9-ZAT10(2x)

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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