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pcDNA3.1 PA-mCit

Plasmid

#113443

Purpose

ProteinA-mCitrine control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321G

Plasmid

#98670

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A321G

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG Luciferase

Plasmid

#98391

Purpose

Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Gateway Cloning

Tags

3xFLAG

Promoter

TRE tight and TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK019-4-tAOX1

Plasmid

#84988

Purpose

Type 4, tAOX, Alcohol oxidase 1 terminator

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 terminator

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-rev

Plasmid

#81219

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_chr12_FAM19A2-up-gRNA-for

Plasmid

#81221

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE7

Plasmid

#79462

Purpose

sgRNA shuttle vector; module 2

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE9

Plasmid

#79465

Purpose

sgRNA shuttle vector; module 3

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE11

Plasmid

#79467

Purpose

sgRNA shuttle vector; module 4E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-BRT17

Plasmid

#67000

Purpose

Expresses malE-BRT17 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-BRT17

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Dros16

Plasmid

#67004

Purpose

Expresses malE-Dros16 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Dros16

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-CLP3.7

Plasmid

#67011

Purpose

Expresses malE-CLP3.7 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-CLP3.7

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pSARSF534-3

Plasmid

#45618

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaEC35-GFP_C

Tags

H6

Expression

Bacterial

Mutation

SYG->CYG in chromophore

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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CAMK1D

Plasmid

#38834

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CAMK1D (Camk1d Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
SI6-IGSF21a

Plasmid

#36141

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

IGSF21a mouse monoclonal antibody (igsf21a Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
SI7-NCAM2

Plasmid

#36142

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

NCAM2 mouse monoclonal antibody (ncam2 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
SI8-IGSF21b

Plasmid

#36143

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

IGSF21b mouse monoclonal antibody (igsf21b Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pOZ-N-FH PSMD14

Plasmid

#27500

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

PSMD14 (PSMD14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

M1V

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only