We narrowed to 17,254 results for: REV
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Plasmid#201431PurposeBacterial protein expression of Dhh1 426-506DepositorInsertDhh1 (DHH1 Budding Yeast)
TagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 426-506 onlyPromotertacAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp1 D82-129-EGFP
Plasmid#201426PurposeBacterial protein expression of Dcp1 del82-129DepositorInsertDcp1 (DCP1 Budding Yeast)
TagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationdeletion of aa 82-129PromotertacAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pat1 241-422-EGFP
Plasmid#201436PurposeBacterial protein expression of Pat1 241-422DepositorInsertPat1 (PAT1 Budding Yeast)
TagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 241-422 onlyPromotertacAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pat1 423-796-EGFP
Plasmid#201437PurposeBacterial protein expression of Pat1 423-796DepositorInsertPat1 (PAT1 Budding Yeast)
TagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 423-796 onlyPromotertacAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Edc3 283-551-EGFP
Plasmid#201434PurposeBacterial protein expression of Edc3 283-551DepositorInsertEdc3 (EDC3 Budding Yeast)
TagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 283-551 onlyPromotertacAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AG
Plasmid#148813PurposeBacterial Expression of ScEap1p_107-122-Y107IA108KS110TDepositorInsertScEap1p_107-122-Y107IA108KS110T (EAP1 Budding Yeast)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
ExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
ExpressionBacterialMutationA822G, A823C, A824TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193314PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
Tags4MYCExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HYG-TET-OFF – ubiquitin – LEU – 4MYC
Plasmid#193316PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4MYC N-terminal tagging
Tags4MYCExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKC1-2GFP-ter-TRP1
Plasmid#184773PurposeIntegration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB106
Plasmid#185065PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domainDepositorInsertNUP1
TagsGSTExpressionBacterialMutationNup1 C-terminal region, truncated so C-terminal h…Available SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
TagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
ExpressionYeastAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only