Addgene

We narrowed to 4,413 results for: Ado;

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gRNA_CloningVector_miRFP670

Plasmid

#163748

Purpose

Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR124.5_tagBFP

Plasmid

#163753

Purpose

gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR124.5

Use

CRISPR

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP C1 SADBs

Plasmid

#67154

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-RFP SADBs

Plasmid

#67153

Purpose

pSG5 containing N-terminally RFP tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-HA SADBs

Plasmid

#67149

Purpose

pSG5 containing N terminally HA tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40, T7

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi SadBs shRNA mouse

Plasmid

#67152

Purpose

pBS/U6 containing shRNA to mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (Brsk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-NFIB-T2A-miRFP670

Plasmid

#187222

Purpose

Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Nuclear Factor I B (NFIB Human)

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_SOX2_HDR

Plasmid

#163752

Purpose

gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SOX2 (SOX2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-mRuby3

Plasmid

#166136

Purpose

Gateway entry vector for mRuby3

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

mRuby3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Type

Empty backbone

Use

Tags

His10

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AbVec2.0-mIghg2b

Plasmid

#127155

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Ighg2b (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AbVec2.1-mIglc2

Plasmid

#127156

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Iglc2 (Iglc2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTER GAMMA-TUBULIN shRNA Human

Plasmid

#87955

Purpose

reduced the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

sh gamma-tubulin (TUBG1 Human)

Use

Tags

No-tag

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene.…

Promoter

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-FOXA1-T2A-eGFP

Plasmid

#182337

Purpose

Expresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Use

Tags

T2A-eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-FOXA1-T2A-miRFP670

Plasmid

#182335

Purpose

Expresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Use

Tags

T2A-miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-Smt3-ccdB

Plasmid

#166147

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Type

Empty backbone

Use

Tags

His10-Smt3

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

DDX5

Use

Tags

His10 and SNAP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

EGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

mRuby3

Use

Tags

Expression

Yeast

Mutation

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge