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We narrowed to 5,630 results for: chia

Showing: 251 - 300 of 5630 results

  9. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  10. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  11. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

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  12. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  16. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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  19. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Use
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

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  20. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    104912-plasmid-map-sequence-id-206066

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  21. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  22. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  23. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  24. pCapVQ329R

    Plasmid
    #183029
    Purpose
    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase variant CapVQ329R
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    glutamine 329 changed to arginine
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183029-plasmid-map-sequence-id-361616

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  25. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  26. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Use
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

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  30. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Use
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

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  32. pAIP4_5M2Y

    Plasmid
    #234193
    Purpose
    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5M2Y
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234193-plasmid-map-sequence-id-464158

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  33. pJK515

    Plasmid
    #72385
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylglycinamide formyltransferase 2
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72385-plasmid-map-sequence-id-133919

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  34. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72399-plasmid-map-sequence-id-133993

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  35. pAIP4_3A7L

    Plasmid
    #234182
    Purpose
    Heterologous protein expression of glycine cleavage system H protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3A7L (GCV3 Escherichia coli K-12)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234182-plasmid-map-sequence-id-464147

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  37. pAIP4_3EYE

    Plasmid
    #234176
    Purpose
    Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3EYE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234176-plasmid-map-sequence-id-464141

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  38. pJK639

    Plasmid
    #72398
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72398-plasmid-map-sequence-id-133991

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  39. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  40. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  41. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  42. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  43. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  44. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  45. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

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  46. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  47. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

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  48. pAIP4_1U8T

    Plasmid
    #234211
    Purpose
    Heterologous protein expression of flagellar motor switch protein FliM in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1U8T (fliM Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234211-plasmid-map-sequence-id-464176

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  49. pAIP4_1EW4

    Plasmid
    #234180
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EW4 (cyaY Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234180-plasmid-map-sequence-id-464145

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  50. pJK640

    Plasmid
    #72467
    Purpose
    Encodes Escherichia coli evgA
    Depositor
    T. J. Kappock
    Insert
    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72467-plasmid-map-sequence-id-134013

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Showing: 251 - 300 of 5630 results