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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 153 results for: A1a

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Showing: 101 - 120 of 153 results

  2. pGEX4T-1-6AlanineHE1

    Plasmid
    #165098
    Purpose
    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
    Depositor
    Arthur Haas
    Insert
    Uba1 (UBA1 Human)
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    None
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165098-plasmid-map-sequence-id-326697

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  3. ADRA1D-Tango

    Plasmid
    #66215
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRA1D (ADRA1D Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  6. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Publication
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

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  7. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid
    #206125
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Publication
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    E1707A
    Promoter
    CMV
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206125-plasmid-map-sequence-id-410308

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  8. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid
    #206124
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Publication
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    E320A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206124-plasmid-map-sequence-id-410294

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  9. pDONR221-SLC4A1_STOP

    Plasmid
    #161346
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC4A1 (SLC4A1 Human)
    Expression
    Mammalian
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    161346-plasmid-map-sequence-id-338285

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  10. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Publication
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

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  11. σ1-mCherry

    Plasmid
    #186578
    Purpose
    Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry
    Depositor
    Stephen Royle
    Publication
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AP1S1 (AP1S1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186578-plasmid-map-sequence-id-368432

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  15. FLAGHis10pFastBacHTA/Serca1

    Plasmid
    #74401
    Purpose
    expression of SERCA in insect cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Tags
    10His and FLAG
    Expression
    Insect
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    polyhedrin
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 153 results