We narrowed to 24,999 results for: Nes

1. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Use

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100852

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTagBFP2-Farnesyl-5

   Plasmid

   #55295

   Purpose

   Localization: Plasma Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBbsr2-4031NES

   Plasmid

   #105241

   Purpose

   A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

   Depositor

   Michiyuki Matsuda

   Article

   Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

   Insert

   AMPK-EV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEND-320_Cacnes_ori

   Plasmid

   #225602

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-4511NES

   Plasmid

   #138375

   Purpose

   Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Booster-ERK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEND-184_Cacnes_mCherry

   Plasmid

   #225610

   Purpose

   Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-164_Cacnes_pFAST

   Plasmid

   #225616

   Purpose

   Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   pFAST
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Kinesin11-N-18

    Plasmid

    #55067

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100857

    Purpose

    AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEND-321_Cacnes_ori

    Plasmid

    #225603

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEND-350_Cacnes_ori

    Plasmid

    #225605

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEND-446_Cacnes_ProA

    Plasmid

    #225609

    Purpose

    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEND-224_Cacnes_sfGFP

    Plasmid

    #225611

    Purpose

    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pP{ELAV-GeneSwitch}

    Plasmid

    #83957

    Purpose

    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

    Depositor

    Haig Keshishian

    Article

    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

    Insert

    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    elav

    Available Since

    Dec. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna nesprin HL

    Plasmid

    #68128

    Purpose

    nesprin headless control

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    nesprin-2 (Syne2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted actin-binding domain

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-Kinesin11-N-18

    Plasmid

    #54137

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. mApple-Farnesyl-5

    Plasmid

    #54899

    Purpose

    Localization: Plasma Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid

    #223508

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y306A; Y384F

    Promoter

    CVM, SP6 and U6

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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