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pN1-CMV-mCherryN-IntN-DrBphP (N23)

Plasmid

#210724

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

N23

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B

Plasmid

#103002

Purpose

non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B3

Plasmid

#103004

Purpose

non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B3 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V2

Plasmid

#127848

Purpose

non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B4

Plasmid

#127849

Purpose

non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.B4 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAV PHP.eB-CAG-Venus-P2A-Tau-RING

Plasmid

#233696

Purpose

AAV expression of Venus-P2A-Tau-RING from CAG promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus-P2A-Tau-RING

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJM101: pHps1 RFP on pCM66T

Plasmid

#74740

Purpose

pCM66T with promoter pHps1 driving RFP expression (maxRFP = BBa_E1010)

Depositor

Mary Lidstrom

Insert

RFP

Promoter

pHps1 (extracted from Methylovorus glucosetrophus…

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJM102: pHps2 RFP on pCM66T

Plasmid

#74741

Purpose

pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)

Depositor

Mary Lidstrom

Insert

RFP (BioBrick part number BBa_E1010)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pHps2 (extracted from Methylovorus glucosetrophus…

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1

Plasmid

#122874

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

human NPHP1 (NPHP1 Human)

Use

Lentiviral

Tags

HA

Promoter

EF1 alpha

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac FPH

Plasmid

#1925

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

polyhomeotic (ph-d, ph-p Fly)

Tags

Flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
KHBD00712

Plasmid

#39741

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18412 (ph-p Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac FPH

Plasmid

#1925

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

polyhomeotic (ph-d, ph-p Fly)

Tags

Flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
KHBD00712

Plasmid

#39741

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18412 (ph-p Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pAAV_BiPVe3_ChR2_mCherry (AAV PHP.eB)

Viral Prep

#213941-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_ChR2_mCherry (#213941). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Tags

mCherry

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only