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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,402 results for: V2

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Showing: 101 - 120 of 1402 results

  4. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    87836-plasmid-map-sequence-id-176310

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  7. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Publication
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113934-plasmid-map-sequence-id-225307

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  10. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    146 Articles
    83480-plasmid-map-sequence-id-423158

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  11. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84 Articles
    89308-plasmid-map-sequence-id-425605

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  13. pRegev_V2

    Plasmid
    #204144
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation.
    Depositor
    Oded Regev
    Publication
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V2 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204144-plasmid-map-sequence-id-405477

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  17. enOgeuIscB with ωRNA-v2

    Plasmid
    #220939
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220939-plasmid-map-sequence-id-443121

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Showing: 101 - 120 of 1402 results