We narrowed to 4,214 results for: bre

1. Anti-QKI-6 [N182/17.1R]

   Plasmid

   #114561

   Purpose

   Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17.1.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-QKI-6 (Human) recombinant mouse monoclonal antibody (QKI Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLIK-Venus-TmiR-G12

   Plasmid

   #25740

   Purpose

   Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 12 miR-shRNA (Gna12 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR2b-PTPN11(D425A,C459S)

   Plasmid

   #215515

   Purpose

   Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   D425A, C459S (Phosphatase inactive)

   Promoter

   none

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pENTR2b-ITGB1(WT)

   Plasmid

   #215450

   Purpose

   Gateway entry vector with integrin beta1 wild-type (WT)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   none

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. CMV-FOXC1-T2A-miRFP670

   Plasmid

   #182336

   Purpose

   Expresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Forkhead Box C1 (FOXC1 Human)
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SAM-DNMT3A-inactive+sgAAVS1_118

   Plasmid

   #213171

   Purpose

   Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_118 (AAVS1 Human)
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDest17_hnRNPA1_FtoW

   Plasmid

   #224254

   Purpose

   Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW

   Depositor

   Tanja Mittag

   Article

   Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

   Insert

   hnRNPA1_FtoW (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F17W, F25W, F31W, F37W, F43W, F62W, F69W, F78W, F…

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CIRC#del

   Plasmid

   #186344

   Purpose

   Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

   Depositor

   Giovanni Blandino

   Article

   Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV2_CAG_oROS-HT(C199S)_WPRE

   Plasmid

   #216415

   Purpose

   Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT(C199S)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Anti-Uncx [N155/9R]

    Plasmid

    #114545

    Purpose

    Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Uncx (Mus musculus) recombinant mouse monoclonal antibody (Uncx Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Anti-TrpC4 [N77/15.1R]

    Plasmid

    #114504

    Purpose

    Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Uncx [N155/9R]

    Plasmid

    #206537

    Purpose

    Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Uncx (Mus musculus) recombinant Mouse monoclonal antibody (Uncx Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-SOX2-T2A-miRFP670

    Plasmid

    #187375

    Purpose

    Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Tags

    T2A-miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV_Syn_Xph18_eGFP_CCR5TC

    Plasmid

    #187443

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV_Syn_Xph15_eGFP_CCR5TC

    Plasmid

    #187442

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176238

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

    Plasmid

    #176150

    Purpose

    N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by POLB gRNA1

    Promoter

    EF1A

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSNA-c029

    Plasmid

    #175471

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    L281R, H288A

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid

    #176087

    Purpose

    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in Lys72 to Ala, and mutation in the PAM…

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-Hygro-EF1A-XL1/BRCT1-Linker-eGFP

    Plasmid

    #176084

    Purpose

    EGFP fused to the C-terminus of a XL1/BRCT1 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XL1/BRCT1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Anti-TRIP8b (constant) [N212/7R]

    Plasmid

    #177514

    Purpose

    Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRIP8b (constant) (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLD-puro-Cn-VA-NHEJ1-S263E

    Plasmid

    #141340

    Purpose

    Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    NHEJ1-S263E (NHEJ1 Human)
    

    Use

    Lentiviral

    Tags

    VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

    Expression

    Mammalian

    Mutation

    S263E

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLD-puro-Cn-VA-NHEJ1-S263A

    Plasmid

    #141341

    Purpose

    Lentiviral vector for expression of human NHEJ1-S263A in mammalian cells

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    NHEJ1-S263A (NHEJ1 Human)
    

    Use

    Lentiviral

    Tags

    VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

    Expression

    Mammalian

    Mutation

    S263A

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQC V5 mKO2 HuR.DelHNS IRES Puro

    Plasmid

    #110389

    Purpose

    γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    V5 and mKO2

    Expression

    Mammalian

    Mutation

    HNS (HuR nuclear-cytoplasmic shuttling sequence) …

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Anti-TRIP8b [N212/7R]

    Plasmid

    #164154

    Purpose

    Mammalian Expression Plasmid of anti-TRIP8b (Rat). Derived from hybridoma N212/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Anti-Iduna/RNF146 [N201/35R]

    Plasmid

    #164157

    Purpose

    Mammalian Expression Plasmid of anti-Iduna/RNF146 (Mouse). Derived from hybridoma N201/35.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Iduna/RNF146 (Mus musculus) recombinant mouse monoclonal antibody (Rnf146 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Anti-ZNF423 [N328B/37R]

    Plasmid

    #140077

    Purpose

    Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPRISM-Fuse-cmlc2-mRFP

    Plasmid

    #117787

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Anti-REEP1/2 [N326D/29R]

    Plasmid

    #114510

    Purpose

    Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Anti-PARIS/ZNF746 [N196/16R]

    Plasmid

    #114475

    Purpose

    Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120295

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Anti-Malin [N85/18.1R]

    Plasmid

    #114513

    Purpose

    Mammalian Expression Plasmid of anti-Malin (Human). Derived from hybridoma N85/18.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Malin (Homo sapiens) recombinant mouse monoclonal antibody (NHLRC1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Anti-Stonin-2 [N346/9R]

    Plasmid

    #114484

    Purpose

    Mammalian Expression Plasmid of anti-Stonin-2 (Human). Derived from hybridoma N346/9.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Stonin-2 (Homo sapiens) recombinant mouse monoclonal antibody (STON2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid

    #110324

    Purpose

    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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41. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid

    #110323

    Purpose

    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Human, Homo sapiens)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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42. pAP1893-1

    Plasmid

    #105248

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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43. 10xHis-ataxin-3 *SIM-HA

    Plasmid

    #89983

    Purpose

    Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    10xHis and HA

    Expression

    Mammalian

    Mutation

    mutated aa 162IFVV to 162AFAA

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. GFP-ataxin-3 *SIM

    Plasmid

    #89979

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 162IFVV to 162AFAA

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. GFP-ataxin-3 *VBM

    Plasmid

    #89976

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 282RKRR to 282HNHH

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEN_TTmiRc2_3xflag_CHEK2

    Plasmid

    #136526

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CHEK2 (CHEK2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid

    #174722

    Purpose

    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    eGFP
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and F2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge