Addgene

We narrowed to 3,035 results for: ALC

Showing: 1181 - 1200 of 3035 results

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Use

Tags

1D4

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pfTRAP

Plasmid

#50048

Purpose

Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

plamodium falciparum TRAP (TRAP Synthetic)

Use

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
IBMc233

Plasmid

#161939

Purpose

Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPhlF

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - CPX

Plasmid

#89620

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Cytosolic Tryparedoxin peroxidase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Use

Tags

GST and thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_Neo_FKBPF36V_mScarlet

Plasmid

#163875

Purpose

Acute Depletion of BRG1-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJH3577

Plasmid

#179612

Purpose

Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Pceh-12

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3558

Plasmid

#179611

Purpose

Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Punc-4

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1b

Plasmid

#164957

Purpose

Zebrafish kcnn1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1b (kcnn1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTL-4

Plasmid

#52033

Purpose

Inducible expression of GST-unc43 in E. coli

Depositor

Meng-Qiu Dong

Article

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

Unc-43 (unc-43 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Expression

Mutation

Promoter

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox c-SPT5_EYFP_FKBPF36V_Neo

Plasmid

#163872

Purpose

Acute Depletion of SPT5-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Spt5 (Supt5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-LGK/csTEM1

Plasmid

#81164

Purpose

pSALECT plasmid with levoglucosan kinase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase

Use

Tags

Codon swapped TEM-1 beta-lactamase (H26 to W290) …

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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