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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,727 results for: BFP

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Showing: 1181 - 1200 of 2727 results

  1. psecBFP2

    Plasmid
    #62230
    Purpose
    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
    Depositor
    Erik Snapp
    Publication
    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;
    Insert
    secBFP2
    Tags
    secbfp2
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  5. pKK-mTagBFP-TEV

    Plasmid
    #105772
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mTagBFP-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    105772-plasmid-map-sequence-id-203361

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  6. mTagBFP2-mEGFP-FRET

    Plasmid
    #58173
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEGFP and mTagBFP2
    Expression
    Mammalian
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  13. EBFP2-Cx30

    Plasmid
    #69022
    Purpose
    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx30 (GJB6 Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69022-plasmid-map-sequence-id-127476

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  14. mTagBFP2-TES-N-14

    Plasmid
    #62786
    Purpose
    Localization: Focal Adhesions, N1 Cloning Vector, Excitation:399, Emission:456
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62786-plasmid-map-sequence-id-173171

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Showing: 1181 - 1200 of 2727 results