We narrowed to 1,255 results for: CFP

1. Lyn FAK biosensor

   Plasmid

   #78299

   Purpose

   FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Lyn Fak biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPT234

   Plasmid

   #127855

   Purpose

   Repressilator 2.0, without degradation tags, integrated triple reporter

   Depositor

   Johan Paulsson

   Article

   Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

   Inserts

   bla
   mSCFP3
   mKate2
   mVenus
   TetR
   LacI
   cI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   First 11aa derived from mCherry as fusion protein

   Promoter

   pAmpR, pL LacO1, pL tetO1, and pR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCerulean-VSVG

   Plasmid

   #11913

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Expression

   Mammalian

   Mutation

   F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSIIbsr-Eevee-ROCK-NES

   Plasmid

   #209918

   Purpose

   A FRET biosensor for ROCK activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   Eevee-ROCK-NES
   

   Use

   Lentiviral

   Tags

   ECFP and YPet

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pSLQ10715

   Plasmid

   #183960

   Purpose

   U6-crOct4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyso-InPAkt

   Plasmid

   #184053

   Purpose

   Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-InPAkt
   

   Tags

   Lysosome-associated membrane protein 1 (LAMP1).

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Twitch-3 pcDNA3

   Plasmid

   #49532

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3
   

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10713

   Plasmid

   #183958

   Purpose

   U6-crSox2-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Sox2
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cerulean-GalT

    Plasmid

    #11930

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmTurquoise-parkin

    Plasmid

    #110945

    Purpose

    Expresses the ubiquitin ligase parkin fused to mTurquoise

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    E3 ubiquitin ligase parkin (PRKN Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-1045C

    Plasmid

    #125005

    Purpose

    Expresses gRNAs targeting hid, eve, and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ10714

    Plasmid

    #183959

    Purpose

    U6-crKlf4-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Klf4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60-mPU.1-ETS

    Plasmid

    #85733

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

    Depositor

    Gregory Poon

    Article

    Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

    Insert

    ETS domain of transcription factor PU.1 (Spi1 Mouse)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCit-Snap25b D179K A199R

    Plasmid

    #53259

    Purpose

    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    SNAP-25B (Snap25 Rat)
    

    Tags

    4aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    D179K and A199R

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

    Plasmid

    #67687

    Purpose

    UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Tags

    6xmyc-NLS-tdTomato-2A

    Mutation

    S159A, S196A

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAFNF-Venus-Actb

    Plasmid

    #169788

    Purpose

    An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Actb (Actb Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. OA-1045B

    Plasmid

    #125004

    Purpose

    Expresses gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. OA-1045E

    Plasmid

    #125007

    Purpose

    Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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