We narrowed to 3,275 results for: ER;

1. pF(UG) CamKII sf iGluSnFR S72A

   Plasmid

   #179726

   Purpose

   Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   iGluSnFR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S72A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW Syt1-C2B R398,399Q IRES GFP

   Plasmid

   #133819

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   R398,399Q

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFSW Syt1-C2B K326,327E IRES GFP

   Plasmid

   #133820

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   K326,327E

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSW Syt1-C2A D230,232N IRES GFP

   Plasmid

   #133824

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   D230,232N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)

   Viral Prep

   #198516-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   EF-1a

   Tags

   mScarlet

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

7. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

   Viral Prep

   #198516-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   EF-1a

   Tags

   mScarlet

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-Neo-Bam p53 wt

   Plasmid

   #16434

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBK.079

    Plasmid

    #187218

    Purpose

    Expresses Eco1 RT and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 Reverse Transcriptase
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115 and T7/lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-mCherry-Stim1

    Plasmid

    #186620

    Purpose

    Third generation lentiviral vector expressing Stim1-wt fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat, Budding Yeast)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Mfn1

    Plasmid

    #141154

    Purpose

    Mammalian expression and labeling mitofusin 1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 1 (MFN1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI pHluorin Syt7

    Plasmid

    #184508

    Purpose

    Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 7 (Syt7 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIP-mCherry-CDMPR

    Plasmid

    #182643

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    CDMPR
    

    Use

    Retroviral

    Tags

    N-terminal ER targeting sequence of hemagglutinin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQCXIP-EGFP-CDMPR

    Plasmid

    #182642

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    CDMPR
    

    Use

    Retroviral

    Tags

    EGFP and N-terminal ER targeting sequence of hema…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R273H

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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