We narrowed to 1,995 results for: Ipp

1. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1

   Plasmid

   #172983

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

   Plasmid

   #204622

   Purpose

   Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

   Plasmid

   #172582

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD2

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

   Plasmid

   #175586

   Purpose

   in vitro transcription of mut RBM8A 3'UTR

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   RBM8A-3'UTR AluY-Sz mut 2598-4066
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120394

   Purpose

   pAAV plasmid expressing Diras2 shRNA2 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Insert

   Diras2 shRNA (Diras2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   mCherry.3XFLAG

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCh-607

   Plasmid

   #92258

   Purpose

   Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCh-633

   Plasmid

   #92253

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   EF1A

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

   Plasmid

   #83458

   Purpose

   Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Amino-acids 4127-4303, S4144D

   Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T-Mst2 SARAH

   Plasmid

   #84298

   Purpose

   Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

   Insert

   Mst2 (STK3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   L443W

   Promoter

   tac

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-Flag-YAP-H122Y

    Plasmid

    #33085

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H122Y

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCMV7.1_3xFLAG-LATS2

    Plasmid

    #172987

    Purpose

    constitutive expression of FLAG-tagged LATS2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-LATS2 (LATS2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. phROSA26-Tet-HA-JSAP1_WT

    Plasmid

    #208772

    Purpose

    Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JSAP1 (MAPK8IP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Promoter

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF(UG) hSyn LAMP1-msGFP (JV012)

    Plasmid

    #179728

    Purpose

    Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    LAMP1 (Lamp1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX NPC1-FLAG-TiD

    Plasmid

    #199748

    Purpose

    Lentiviral construct to stably express NPC1 TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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