Addgene

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EC70190

Plasmid

#209451

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_HALMA

Plasmid

#204241

Purpose

Bacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_HALMA (mdh Haloarcula marismortui)

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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EC67908

Plasmid

#211795

Purpose

Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas12a, GR-GRF-GIF

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pQF-lacZ

Plasmid

#48094

Purpose

Cumate-inducible expression of lacZ

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Insert

E. coli lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMpGWB105-MpTubulin

Plasmid

#100588

Purpose

Binary vector for expression of Citrine-fused MpTubulin beta 3 (Citrine-MpTubulin) to visualize microtubules in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Citrine-MpTubulin

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

HA tag

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

SYFP2

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pR-mClover3

Plasmid

#228605

Purpose

Mammalian expression of mClover3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DIG70 (T7->Rci; 11bc)

Plasmid

#68835

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 11 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 11 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

myc tag

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pL0_AC_p45582

Plasmid

#154036

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter 45582

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tNub

Plasmid

#154039

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Nub

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_AC_pSVP

Plasmid

#154040

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter SVP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tPbt

Plasmid

#154043

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Pbt

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pR-miRFP670

Plasmid

#228612

Purpose

Mammalian expression of miRFP670, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pR-EBFP2

Plasmid

#228595

Purpose

Mammalian expression of EBFP2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pReceiver

Plasmid

#228594

Purpose

Mammalian expression of a single fluorescent protein, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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