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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNR291

Plasmid

#79555

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

GFPmut3b
mTagBFP
mRFP

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ATG5 sgRNA

Plasmid

#207555

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

AACTTGTTTCACGCTATATC

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5 PMS2-134

Plasmid

#16476

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

PMS2 1-134 (PMS2 Human)

Expression

Mammalian

Mutation

Codons 1-134

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJK667

Plasmid

#71690

Purpose

Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

Depositor

T. J. Kappock

Article

Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

Insert

acetate kinase (ackA Escherichia coli)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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Banshee-Sh-Ctrl

Plasmid

#85587

Purpose

Control hairpin sequence used in gene knockdown experiements

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

sh-Ctrl

Use

Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Cdc12-Cdc11

Plasmid

#131162

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

6his-Cdc12 + Cdc11

Use

Bicistronic

Tags

Cdc11: S-Tag and Cdc12: 6his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10

Plasmid

#131160

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10

Use

Bicistronic

Tags

Cdc10: S Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BssgRNA

Plasmid

#121957

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 SMR1747

Plasmid

#28029

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutS (mutS E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Tags

c-myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
His6-mCherry-Flag-LplA W37V

Plasmid

#248802

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and C55I

Plasmid

#248807

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and C55I

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and R337P

Plasmid

#248805

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and R337P

Plasmid

#248817

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and C55M

Plasmid

#248819

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and C55M

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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