We narrowed to 19,518 results for: Tec

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB444

Plasmid

#74830

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

Plasmid

#191159

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP:EGFP.s2:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

Plasmid

#194217

Purpose

Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

UAS-ybTATA-tagBFP; PGK-mCitrine

Use

Lentiviral

Promoter

GAL4 UAS-ybTATA; PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadCBEd

Plasmid

#193841

Purpose

Express TadCBEd (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEd

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB518

Plasmid

#74860

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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ER-HA-mNG21-10

Plasmid

#141165

Purpose

Split Fluorescent Reporter for ER Retrograde Trafficking

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

ER-HA-mNG21-10-KDEL

Use

Lentiviral

Tags

Signal Sequence, KDEL and HA

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

Ef1a

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57 APEX

Plasmid

#40306

Purpose

A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

pea APEX (Synthetic)

Use

Unspecified

Mutation

K14D, W41F, E112K (relative to wild-type)

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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