200,238 results

1. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-KIR3DS1-COMP5AP-AviTag-9xHis

   Plasmid

   #157323

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR3DS1 (KIR3DS1 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM60-FLAG NUFIP1

   Plasmid

   #110950

   Purpose

   expresses mammalian FLAG-NUFIP1

   Depositor

   David Sabatini

   Article

   Wyant et al Science. 2018 May 18;360(6390):751-758.

   Insert

   NUFIP1 (NUFIP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-32a(+)-TGFB3m

   Plasmid

   #120289

   Purpose

   Produce human recombinant mTGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β3
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-BBS1

   Plasmid

   #218711

   Purpose

   Expresses N-terminally EGFP-tagged BBS1 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   BBS1 (BBS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-mito-mCer

   Plasmid

   #185602

   Purpose

   Lentiviral construct for expression of mCerulean targeted to mitochondria

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   mito-mCer
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDblet

   Plasmid

   #8848

   Depositor

   Joel Huberman

   Article

   Brun et al Gene. 1995 Oct 16. 164(1):173-7.

   Insert

   ARS doublet
   

   Use

   Yeast cloning vector

   Expression

   Yeast

   Mutation

   This vector contains, between AatII sites, a dire…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDblet

   Plasmid

   #8848

   Depositor

   Joel Huberman

   Article

   Brun et al Gene. 1995 Oct 16. 164(1):173-7.

   Insert

   ARS doublet
   

   Use

   Yeast cloning vector

   Expression

   Yeast

   Mutation

   This vector contains, between AatII sites, a dire…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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